Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides
نویسندگان
چکیده
منابع مشابه
Comparison of biological activities of human antithrombins with high-mannose or complex-type nonfucosylated N-linked oligosaccharides
The structure of the N-linked oligosaccharides attached to antithrombin (AT) has been shown to affect its anticoagulant activity and pharmacokinetics. Human AT has biantennary complex-type oligosaccharides with the unique feature of lacking a core fucose, which affects its biological activities by changing its heparin-binding affinity. In human plasma, AT circulates as a mixture of the α-form b...
متن کاملN-Linked High Mannose-type Oligosaccharides
Incubation of Crithidia fasciculata cells with [U-’“C] glucose led to the synthesis of Man-P-dolichol but not of Glc-P-dolichol. The main and largest dolicho1-P-Plinked oligosaccharide formed was Man7GlcNAcz whether labeling was performed in 5 mM sodium pyruvate or 5.5 mM glucose. The protein-linked, endo-j3-N-acetylglucosaminidase H-sensitive oligosaccharides isolated from mature glycoprote...
متن کاملComparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types.
The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it is still unclear how differences in the N-linked oligosaccharide structures imp...
متن کاملGlucose persistence on high-mannose oligosaccharides selectively inhibits the macroautophagic sequestration of N-linked glycoproteins.
The macroautophagic-lysosomal pathway is a bulk degradative process for cytosolic proteins and organelles including the endoplasmic reticulum (ER). We have previously shown that the human colonic carcinoma HT-29 cell population is characterized by a high rate of autophagic degradation of N-linked glycoproteins substituted with ER-type glycans. In the present work we demonstrate that glucosidase...
متن کاملHigh-mannose dolichol-linked oligosaccharides in infantile ceroid-lipofuscinosis.
acid. Tryptic peptides were prepared from the oligosaccharide-containing CNBr peptide (see below) by incubation at 2 mg/ml in 2% (w/v) ammonium bicarbonate, pH 8.0, with trypsin (Worthington TRTPCK; E:S of I : 100) at 25°C for 3 h, followed by freeze-drying. The peptides were dissolved in 0.1% (v/v) trifluoracetic acid and separated on a 4.6 mmx250 mm C-18 reverse-phase column (Vydac 201TP 104;...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 2016
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/cww001